Antioxidant Properties of ADJ6 and ITS Effect on Isolated Human Erythrocyte Ghost Membrane

groupview

Abstract
Free Radicals and Antioxidants,2016,6,2,133-138.
Published:Jan/2016
Type:Original Article

Antioxidant Properties of ADJ6 and ITS Effect on Isolated Human Erythrocyte Ghost Membrane

Anand Duraiswamy1, Devanand Shanmugasundaram1, Changam Sheela Sasikumar1*, Sanjay M. Cherian2, Kotturathu Mammen Cherian2

1Department of Cellular and Molecular Biochemistry, Frontier Mediville (A Unit of Frontier Lifeline and Dr. K. M. Cherian Heart Foundation), Affiliated to University of Madras, Chennai, Tamil Nadu, INDIA.

2Department of Cardiothoracic Surgery, Frontier Lifeline Hospital, Chennai, Tamil Nadu, INDIA.

Abstract:

Introduction: Oxidative stress is one of the major causes for many adverse complications especially in disorders such as diabetes, cardiovascular diseases, cancer, etc. Many studies have proven that erythrocytes are affected during these conditions and oxidative stress plays a critical role in damaging the membrane integrity of erythrocytes by inducing lipid peroxidation causing instability to membrane in turn affecting the ion transport mechanisms. Objective and methods: The present study focuses to study the ameliorative effect of ADJ6 against oxidative stress on human erythrocytes. The membrane stabilization activity, Inhibition of lipid peroxidation and the effect against Ouabain induced Na+ K+ ATPase activity was assessed. Further, in vitro antioxidant activity of ADJ6 was also determined. Results and Discussion: DPPH radical scavenging activity, metal chelating and H2O2 radical scavenging activity were IC50=37.9 ± 0.22 μg/ml, IC50=53.95 ± 0.337 μg/ml and IC50=69.5 ± 0.628 μg/ml respectively. ADJ6 showed an EC50=90.83 ± 1.905 μg/ml against hypotonicity induced lysis of erythrocytes. H2O2 induced Lipid peroxidation of ghost membrane was also inhibited by ADJ6 (IC50=84.5 ± 2.613 μg/ml). Ouabain induced sodium potassium ATPase activity increased with the treatment of ADJ6 in a dose dependent manner. This proves that ADJ6 is effective in stabilizing the membrane potential of erythrocyte membrane. The scavenging activity in vitro also proves that ADJ6 is effective against free radicals. Conclusion: Though exact mechanism hitherto unknown, it is evident that ADJ6 stabilizes erythrocyte membrane activity. Further studies will be conducted in order to prove the mechanism of action of ADJ6 against of free radicals.

About Journal

Founded:  In 2004, as the PHCOG.NET – a non-profit private organization dedicated to Natural Products Research leading to develop promising drugs. Our main mission is to make information on herbal drug research readily available in different formats to suit the individual needs. About Journal : Free Radicals and Antioxidants [ISSN: 2231-2536] is an online peer reviewed publication dedicated to publishing novel, original findings in the field of free radical biology and both natural and synthetic antioxidants. Free radicals, which are defined as molecules containing one or more unpaired electrons in their outmost atomic or molecular orbital have emerged as double edged swords.The Free Radicals and Antioxidants Journal aims to foster the dissemination of scientific information by publishing manuscripts that provide new insights into the biology of free radicals, including their generation, degradation, modulation, role in cellular function and cell signaling. The submission of uninvited expert reviews and research articles in all areas of Free Radical Biology and Antioxidants are also welcomed. Free Radicals and Antioxidants publishes full research papers presenting original, high quality research, critical review articles providing comprehensive analysis of research development within a defined area and editorial commentaries on key topical issues in Free Radical and Antioxidant Biology.
Distinctions:  The most widely read, cited, and known Free Radicals and Antioxidants and website is well browsed with all the articles published. More than 20,000 readers in nearly every country in the world each month
Editorial Board
Subjects Covered : Natural Products, Free Radical Biology, Antioxidants
ISSN : 2231-2536 ; Frequency : Half Yearly - Rapid at a time publication (2 issues/year)
Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online  publication is 45 days.
Open Access Journal: Free Radicals and Antioxidants is an open access journal, which allows authors to fund their article to be open access from publication.