Comparative Assessment of the Anti-oxidant and Anti-clastogenic Activity of Morus alba Leaves


Free Radicals and Antioxidants,2017,7,1,123-127.
Published:August 2016
Type:Original Article
Author(s) affiliations:

Anjali Raj1*, Kenganora Mruthunjaya2, Subba Rao Venkata Madhunapantula3 and Santhepete Nanjundaiah Manjula1*

1Department of Pharmacology, JSS College of Pharmacy, JSS University, Mysuru, Karnataka, INDIA.

2Department of Pharmacognosy, JSS College of Pharmacy, JSS University, Mysuru, Karnataka, INDIA.

3Department of Biochemistry, Center of Excellence in Molecular Biology and Regenerative Medicine (CEMR), JSS Medical College, Mysuru, Karnataka, INDIA.


Objective: The present study was conducted to investigate the antioxidant and anti-clastogenic activity of fractions extracted from Morusalba (Moraceae) leaves. Methods: The leaves of Morus alba were sequentially extracted using n-Hexane, Petroleum Ether, Ethyl Acetate, Methanol and Water, and Total phenolic content was determined using Folin-Ciocalteau (FC) reagent. The anti-oxidant property of each fraction was determined using 2-Deoxy-D-ribose, 2, 2-Diphenyl-1-picryl-hydrazyl (DPPH) and Hydroxyl radical scavenging assay. Furthermore, anti-clastogenic potential of these fractions was determined using calf-thymus DNA in the presence of Fenton’s reagent followed by analyzing the samples by agarose gel electrophoresis. Results: All fractions showed the presence of varied amounts of total phenols and exhibited anti-oxidant activity as determined by scavenging the corresponding radicals. Among these fractions the methanol fraction (MEMA) showed the maximum phenol content as well as anti-oxidant activity. In addition MEMA could inhibit the breakage of DNA strands (anti-clastogenic activity) compared to other fractions tested. Discussion: Results of this study suggests that MEMA is likely to possess better antioxidant and anti-clastogenic activity.