Anthocyanin Isolation from Berberis integerrima Bunge Fruits and Determination of their Antioxidant Activity

groupview

Abstract
Free Radicals and Antioxidants,2018,8,1,01-05.
Published:October 2017
Type:Original Article

Anthocyanin Isolation from Berberis integerrima Bunge Fruits and Determination of their Antioxidant Activity

Fatemeh Farmani1, Mahmoodreza Moein1,2, Mohammad Javad Khoshnood3, Zahra Sabahi1*

1Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, IRAN.

2Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IRAN.

3Department of Toxicology and Pharmacology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IRAN.

Abstract:

Background: Anthocyanins are famous members of flavonoid group with multifaceted effect including anti-inflammatory, anti-oedema activities, antioxidant and antitumor activity. Berberis integerrima is candidate in treatment of gastrointestinal diseases, bleeding, fever, malaria and hepatitis. The aim of this study is to isolate anthocyanin of Berberis integerrima Bunge fruits (AFBI) and measurement of free-radical-scavenging activities by different methods. Materials and Methods: AFBI isolated by column chromatography. Anthocyanin content was measured by PH-differential method and antioxidant activities of AFBI quantified by various methods (DPPH, FRAP, ABTS) as well as lipid peroxidation. Results: Anthocyanin content is 14.36 ± 0.33 mg/g before purification by column and 34.51 ± 0.42 mg/g in AFBI. IC50 of anthocyanin fraction before loading on column are 471.06 ± 1.8 μg/ml, 65.98 ± 0.66 μg/ml and 495.5 ± 1 μg/ml for DPPH, FRAP and ABTS assay, respectively. These IC50 after loading on column are 66 ± 0.04 μg/ml, 11 ± 0.16 and 60 ± 2.1 μg/ml for DPPH, FRAP and ABTS assay, respectively. Nitric oxide scavenging values are 53 ± 0.5% and 97.04 ± 0.69% before and after using column for anthocyanin purification. The percentages of lipid peroxidation inhibition of AFBI are about 39% in first day and 95% after 10 days. Conclusion: According to results, mentioned method is efficient for anthocyanin purification and antioxidant activity increased significantly after loading sample on this column. Berberis integerrima fruits can be noticed as proper source of anthocyanin which able to scavenge different free radicals and protective agent against lipid peroxidation.

About Journal

Founded:  In 2004, as the PHCOG.NET – a non-profit private organization dedicated to Natural Products Research leading to develop promising drugs. Our main mission is to make information on herbal drug research readily available in different formats to suit the individual needs. About Journal : Free Radicals and Antioxidants [ISSN: 2231-2536] is an online peer reviewed publication dedicated to publishing novel, original findings in the field of free radical biology and both natural and synthetic antioxidants. Free radicals, which are defined as molecules containing one or more unpaired electrons in their outmost atomic or molecular orbital have emerged as double edged swords.The Free Radicals and Antioxidants Journal aims to foster the dissemination of scientific information by publishing manuscripts that provide new insights into the biology of free radicals, including their generation, degradation, modulation, role in cellular function and cell signaling. The submission of uninvited expert reviews and research articles in all areas of Free Radical Biology and Antioxidants are also welcomed. Free Radicals and Antioxidants publishes full research papers presenting original, high quality research, critical review articles providing comprehensive analysis of research development within a defined area and editorial commentaries on key topical issues in Free Radical and Antioxidant Biology.
Distinctions:  The most widely read, cited, and known Free Radicals and Antioxidants and website is well browsed with all the articles published. More than 20,000 readers in nearly every country in the world each month
Editorial Board
Subjects Covered : Natural Products, Free Radical Biology, Antioxidants
ISSN : 2231-2536 ; Frequency : Half Yearly - Rapid at a time publication (2 issues/year)
Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online  publication is 45 days.
Open Access Journal: Free Radicals and Antioxidants is an open access journal, which allows authors to fund their article to be open access from publication.