@article{Ernst Kerche-Silva_Gomes Silva Morais Cavalcante_Silva Danna_Silva Gomes_Moratto Carrara_Lourenço Cecchini_Yoshihara_Eloizo Job_2016, title={Free-radical Scavenging properties and Cytotoxic Activity Evaluation of Latex C-serum from Hevea brasiliensis RRIM 600}, volume={7}, url={https://antiox.org/index.php/fra/article/view/118}, DOI={10.5530/fra.2017.1.16}, abstractNote={<p><strong>Background:</strong> There are few studies exploring the antioxidant properties of the latex C-serum of the <em>Hevea brasiliensis</em> latex and their possible use for therapeutic application.<strong> Objective:</strong> This study investigated the<em> in vitro</em> antioxidant activity of the latex C-serum of<em> H. brasiliensis</em> and assessed the cytotoxicity of this fraction in two human cell lines derived from normal tissue (MRC-5 and CCD 1059sk. Chemical characterization was also assessed. <strong>Methods:</strong> Latex from <em>H. brasiliensis</em> was centrifuged and the C-serum phase was tested for<em> in vitro</em> free radical scavenging assays, such as hydroxyl and nitric oxide radicals, hydrogen peroxide and total antioxidant capacity by the phosphomolibdate method. Latex C-serum was also investigated for cytotoxic properties by the MTT test. Latex C-serum was chemical characterized by Infrared Spectroscopy Fourier Transform (FTIR). <strong>Results:</strong> Latex C-serum effectively scavenged hydroxyl and nitric oxide radicals and hydrogen peroxide and presented potent antioxidant activity. The results were compared to ascorbic acid, a standard antioxidant. The cytotoxicity evaluation of latex C-serum demonstrated cell line dependency and for CCD 1059sk cells derived from skin fibroblasts, latex C-serum was not cytotoxic. FTIR showed the presence of proteins in latex C-serum. <strong>Conclusion:</strong> Latex C-serum from <em>Hevea brasiliensis</em> presented strong antioxidant potential and these results openthe possibility of further studies aiming the use of latex C-serum as treatment for skin diseases related to reactive oxygenspecies or skin preventive pharmacological formulations.</p>}, number={1}, journal={Free Radicals and Antioxidants}, author={Ernst Kerche-Silva, Leandra and Gomes Silva Morais Cavalcante, Dalita and Silva Danna, Caroline and Silva Gomes, Andressa and Moratto Carrara, Iriana and Lourenço Cecchini, Alessandra and Yoshihara, Eidi and Eloizo Job, Aldo}, year={2016}, month={Sep.}, pages={107–114} }