@article{Angel T. Alex_Niranga Hansini Nawagamuwa_Alex Joseph_Josyula V. Rao_Jesil A. Mathew_Nayanabhirama Udupa_2012, title={In vitro anti-cancer and anti-oxidant activity of different fractions of Diospyros peregrina unripe fruit extract}, volume={2}, url={https://antiox.org/index.php/fra/article/view/256}, DOI={10.5530/ax.2012.4.8}, abstractNote={<p><strong>Background:</strong> <em>Diospyros peregrina</em> (Ebenaceae) is a medium-sized evergreen tree having ethnomedicinal significance as an aphrodisiac, astringent, bactericide and for the treatment of diarrhoea, cholera, dysentery, fever, malaria and diabetes.<strong> Objectives:</strong> To evaluate the anti-oxidant and anti-cancer activity of different extracts and fractions of matured unripe fruits of<em> Diospyros peregrina</em> on various <em>in vitro</em> models. <strong>Materials and Methods:</strong> Anti-oxidant and free radical scavenging activity of various extracts and fractions were determined by DPPH, ABTS and Alkaline DMSO methods. <em>In vitro</em> cytotoxicity of different extracts and fractions of matured unripe fruits of <em>Diospyros peregrina</em> was evaluated on MCF-7 and Hep-G2 cell lines by MTT and SRB methods. <strong>Results:</strong> Among the extracts and fractions studied for antioxidant activity n-butanol fraction (NBF) and aqueous extract (AE) showed potential scavenging effect against DPPH, ABTS and superoxide free radicals. <em>In vitro</em> cytotoxicity assays of extracts and fractions by MTT and SRB methods revealed that dichloromethane fraction (DCMF) exhibited maximum cytotoxicity on both MCF-7 and Hep G2 cell lines. <strong>Conclusion:</strong> The higher anti-oxidant potential of n-butanol fraction (NBF) and aqueous extract (AE) may be due to the presence of phenolic compounds, flavonoids or flavonoid glycosides. Dichloromethane fraction (DCMF) exhibited maximum cytotoxicity on both MCF-7 and Hep G2 cell lines. The cytotoxic activity of DCM fraction may be attributed due the presence of triterpenoids.</p>}, number={4}, journal={Free Radicals and Antioxidants}, author={Angel T. Alex and Niranga Hansini Nawagamuwa and Alex Joseph and Josyula V. Rao and Jesil A. Mathew and Nayanabhirama Udupa}, year={2012}, month={Dec.}, pages={45–49} }