@article{Chikezie P.C_Akuwudike A. R_Chikezie C. M_2012, title={Membrane stability and methaemoglobin content of human erythrocytes incubated in aqueous leaf extract of Nicotiana tabacum product}, volume={2}, url={https://antiox.org/index.php/fra/article/view/260}, DOI={10.5530/ax.2012.4.10}, abstractNote={<p>The present study seeks to ascertain membrane stability and methaemoglobin content (%MetHb) of human erythrocytes suspended in aqueous leaf extract of <em>N. tabacum</em>. Erythrocyte membrane stability (%EMS) and %MetHb were measured by spectrophotometric method. Erythrocytes incubated in the five experimental concentrations of <em>N. tabacum</em> exhibited higher tendency to haemolyze in phosphate buffered saline solution [PBS] > 0.54 g/100 mL, whereas erythrocytes incubated in aqueous extracts of [<em>N. tabaccum</em>] = 0.4 and 0.8 mg/100 mL showed relatively lower level of haemolysis in [PBS] < 0.54 g/100 mL, compared with the control samples. Experimental [<em>N. tabacum</em>] = 2.0 mg/100 mL caused destabilization of erythrocyte membrane by 13.3%. %MetHb of control and erythrocytes treated with aqueous extracts of <em>N. tabacum</em> ranged between 0.58 ± 0.09 and 1.89 ± 0.12%. Specifically, erythrocytes treated with [<em>N. tabacum</em>] = 0.4 mg/100 mL gave %MetHb = 0.59 ± 0.08; p < 0.05. Experimental [<em>N. tabacum</em>] = 2.0 mg/100 mL engendered a paradoxical reduction in erythrocyte %MetHb = 0.58 ± 0.09; p > 0.05. The medium negative correlation (r = −0.62421) between MCF and %MetHb was an indication that N. tabacum destabilizing effect, exemplified by [<em>N. tabacum</em>] = 0.2 mg/100 mL; %MetHb = 0.58 ± 0.09; %EMS = −13.3, was not an oxidative process, but by mechanical perturbation by constituents of the plant extract on erythrocyte membrane structural components. Erythrocyte %MetHb in the presence of the five experimental [<em>N. tabacum</em>] = 0.4 − 2.0 mg/100 mL did not cause toxic methaemoglobinemia <em>in vitro</em>.</p>}, number={4}, journal={Free Radicals and Antioxidants}, author={Chikezie P.C and Akuwudike A. R and Chikezie C. M}, year={2012}, month={Dec.}, pages={56–61} }