@article{Narayan L_Rai V_2015, title={Anti-HIV-1 Activity of Ellagic acid Isolated from Terminalia paniculata}, volume={6}, url={https://antiox.org/index.php/fra/article/view/70}, DOI={10.5530/fra.2016.1.12}, abstractNote={<p><strong>Objective:</strong> The present investigation evaluates the potential Anti-HIV-1 and antioxidant activity of the methanol extract of the leaves of <em>Terminalia paniculata</em>. This search for inhibitors of Anti-HIV-1 Integrase and Protease from medicinal plants of Western Ghats, India led to the isolation of Ellagic acid from the methanol extract of <em>T. paniculata</em> leaves.<strong> Methodology:</strong> The<em> in vitro</em> antioxidant activity was determined by different standard methods such as 1,1-diphenyl-2-picryl hydrazyl radical (DPPH), metal chelation, superoxide radical scavenging and lipid peroxidation assays. The<em> in vitro</em> anti- HIV-1 enzyme inhibition assays were carried out by standard protocols using Multiplate integration assay. The structure of Ellagic acid was elucidated on the basis of NMR, LC-MS and FTIR studies. <strong>Results:</strong> Ellagic acid has shown excellent <em>in vitro</em> anti-HIV-1 activity in the enzyme inhibition assays carried out with an IC<sub>50</sub> value of 8.7 μm and 70.4 μm against HIV-1 Integrase and HIV-1 Protease enzymes respectively. For the antioxidant assays carried out Ellagic acid has shown scavenging potential of 92.2 ± 0.03% of DPPH radical, 88.1 ± 0.41% chelation of metal ions, 68.02 ± 0.12% scavenging of superoxide anion radical and 85.52 ± 0.06% inhibition of lipid peroxidation all at a concentration of 40 ppm. <strong>Conclusion:</strong> The isolated bioactive molecule, Ellagic acid has shown highly efficient<em> in vitro</em> anti-HIV and antioxidant activity. The present study happens to be the first report on the HIV-1 inhibition ability of <em>T. paniculata</em>.</p>}, number={1}, journal={Free Radicals and Antioxidants}, author={Narayan L, Chaitra and Rai V, Ravishankar}, year={2015}, month={Aug.}, pages={101–108} }