TY - JOUR AU - Ngoc Boi, Vu AU - Xuan Cuong, Dang AU - Thi Khanh Vinh, Phan PY - 2016/09/01 Y2 - 2024/03/28 TI - Effects of extraction conditions over the phlorotannin content and antioxidant activity of extract from brown algae Sargassum serratum (Nguyen Huu Dai 2004) JF - Free Radicals and Antioxidants JA - Free Rad. Antiox. VL - 7 IS - 1 SE - Original Article DO - 10.5530/fra.2017.1.17 UR - https://antiox.org/index.php/fra/article/view/120 SP - 115-122 AB - <p><strong>Introduction:</strong> This study focused on the discussion of effect of various extraction conditions for phlorotannin content and antioxidant activities extracted from brown algae <em>Sargassum serratum.</em> The algae was grown in the tropical coastal areas of Vietnam. <strong>Methods:</strong> The various extraction conditions include the following parameters: temperature (30-800C), maceration time (1/2–2,5 hours; 8-48 hrs), the ratio of solvent to material (10:1–70:1 (v/w)), pH (2–8), various kind of solvents (ethanol, acetone, chloroform, ethyl acetate and n-hexan) and solvent concentrations. Phlorotannin content, antioxidant activities, and some phytochemical compositions of <em>Sargassum serratum</em> were evaluated. <strong>Results:</strong> The highest phlorotannin content and antioxidant activities was expressed when extracting in the following conditions: 100% ethanol solvent at 500C in 32 hours with the ratio of solvent to material of 40/1 (v/w), pH 7 and one-step extraction. The extract contained flavonoid, terpenoid, alkaloid, fatty and oil. DPPH free radical scavenging of the extract was 87.53%.<strong> Conclusion:</strong> Phlorotannin content and antioxidant activities extracted from <em>Sargassum serratum</em> was depended on the extracting conditions. The condition of extraction for antioxidant phlorotannin and the chemical composition of extract was determined. Brown algae <em>Sargassum serratum</em> has high antioxidant phlorotannin content. A total of 6 compounds were identified from the extract of <em>Sargassum serratum</em>.</p> ER -