TY - JOUR AU - Raj, Anjali AU - Mruthunjaya, Kenganora AU - Venkata Madhunapantula, Subba Rao AU - Manjula, Santhepete Nanjundaiah PY - 2016/09/01 Y2 - 2024/03/29 TI - Comparative Assessment of the Anti-oxidant and Anti-clastogenic Activity of Morus alba Leaves JF - Free Radicals and Antioxidants JA - Free Rad. Antiox. VL - 7 IS - 1 SE - Original Article DO - 10.5530/fra.2017.1.18 UR - https://antiox.org/index.php/fra/article/view/122 SP - 123-127 AB - <p><strong>Objective:</strong> The present study was conducted to investigate the antioxidant and anti-clastogenic activity of fractions extracted from <em>Morusalba</em> (Moraceae) leaves.<strong> Methods:</strong> The leaves of <em>Morus alba</em> were sequentially extracted using n-Hexane, Petroleum Ether, Ethyl Acetate, Methanol and Water, and Total phenolic content was determined using Folin-Ciocalteau (FC) reagent. The anti-oxidant property of each fraction was determined using 2-Deoxy-D-ribose, 2, 2-Diphenyl-1-picryl-hydrazyl (DPPH) and Hydroxyl radical scavenging assay. Furthermore, anti-clastogenic potential of these fractions was determined using calf-thymus DNA in the presence of Fenton’s reagent followed by analyzing the samples by agarose gel electrophoresis.<strong> Results:</strong> All fractions showed the presence of varied amounts of total phenols and exhibited anti-oxidant activity as determined by scavenging the corresponding radicals. Among these fractions the methanol fraction (MEMA) showed the maximum phenol content as well as anti-oxidant activity. In addition MEMA could inhibit the breakage of DNA strands (anti-clastogenic activity) compared to other fractions tested. <strong>Discussion:</strong> Results of this study suggests that MEMA is likely to possess better antioxidant and anti-clastogenic activity.</p> ER -