TY - JOUR AU - Vinay Kant, AU - Madhuri Mehta, AU - Chandresh Varshneya, PY - 2012/12/29 Y2 - 2024/03/28 TI - Antioxidant potential and total phenolic contents of seabuckthorn (Hippophae rhamnoides) pomace JF - Free Radicals and Antioxidants JA - Free Rad. Antiox. VL - 2 IS - 4 SE - Original Article DO - 10.5530/ax.2012.4.14 UR - https://antiox.org/index.php/fra/article/view/267 SP - 79-86 AB - <p><strong>Introduction:</strong> Seabuckthorn (<em>Hippophae rhamnoides</em> L.,<em> Elaeagnaceae</em>) is an important medicinal resource found in abundance in Indian subcontinent especially the North Western Himalayan regions and all parts of the plant are rich in bioactive substances. <strong>Methods:</strong> The antioxidative activity of three different extracts (100% Methanolic, 70% Methanolic, 100% Aqueous) of pomace were evaluated by various<em> in vitro</em> methods such as ABTS, DPPH, nitric oxide, reducing power, superoxide and hydroxyl radical. Total phenolic contents were determined spectrophometrically. <strong>Results:</strong> The 100% methanolic extract showed higher recovery (11%) and total phenolic contents (302.72 ± 4.17 mg of GAE/gm of extract), as compared to the other extracts. All the extracts were able to scavenge different <em>in vitro</em> radicals i.e. ABTS, DPPH, superoxide, hydroxyl and nitric oxide radicals, in a concentration dependent manner. The IC<sub>50</sub> values, determined by using linear regression analysis, revealed that 100% methanolic extract was better scavenger of ABTS, DPPH and hydroxyl radicals. However, 70% methanolic extract was better scavenger of superoxide and nitric oxide radicals. The reducing power of the extracts was also found in a dose dependent manner and was higher in methanolic extracts (100% and 70%). <strong>Conclusion:</strong> On the basis of present study, it could be concluded that pomace of seabuckthorn has pronounced antioxidant properties and can be incorporated in the feed as a nutraceutical after conducting safety and toxicological studies.</p> ER -