TY - JOUR AU - Roy, Anirban AU - Ghosh, Satyabrata AU - Chakraborty, Runu PY - 2019/04/01 Y2 - 2024/03/28 TI - Comparative Research on Anti-Inflammatory Effects of Different Dietary Antioxidants on Alcohol-Induced Damage in Gastric Cells JF - Free Radicals and Antioxidants JA - Free Rad. Antiox. VL - 9 IS - 1 SE - Original Article DO - 10.5530/fra.2019.1.4 UR - https://antiox.org/index.php/fra/article/view/28 SP - 16-21 AB - <p><strong>Objective:</strong> Antioxidants prevent ill-effects of free radicals through regulation of different proand anti-inflammatory enzymes of the cell which have association with the pathophysiology of alcohol-induced gastropathy. This study was aimed to explore the mechanism in gastric adenocarcinoma (AGS) cell line and protection by dietary antioxidants e.g. catechin, resveratrol, quercetin, curcumin and 6-gingerol. <strong>Methods:</strong> AGS cells were exposed to 2.5% ethanol for varied time span in presence and absence of antioxidants. Cytotoxicity was assessed by MTT assay and annexin V-propidium iodide staining. The damages in cellular morphology were observed by DAPI and EtBr-acridine orange staining. Changes in ROS generation were examined through confocal microscopy. Western blots were performed using cell extracts to investigate the changes in expression level of COX-1, -2, catalase, superoxide dismutase (SOD), iNOS, MMP-9 and TIMP-1. Immunofluorescence study was done to cross check the expression of COX-2. <strong>Results:</strong> A concentration-dependent cytotoxicity was observed in the cell viability on ethanol exposure which was reduced by antioxidant treatment. Antioxidants reduced the inflammation by downregulating the expression of COX-2, iNOS and MMP-9 and by upregulating the expressions of catalase, SOD and TIMP-1 significantly. Catechin and quercetin demonstrated most prominent cytoprotection amongst the five, followed by resveratrol. These results were corroborated with MTT assay, DAPI and EtBr-acridine orange staining. Curcumin and 6-gingerol did not show any significant effect. <strong>Conclusion:</strong> Dietary antioxidants protect AGS cells from oxidative stress by maintaining the homeostasis between oxidant-antioxidant and protease-antiprotease ratio.</p> ER -