TY - JOUR AU - Parasuraman, Subramani AU - Raipan, Vanishya A/P PY - 2021/07/30 Y2 - 2024/03/28 TI - In vitro Antioxidant Activity of Methanolic and Ethanolic Extracts of seeds of Macrotyloma uniflorum JF - Free Radicals and Antioxidants JA - Free Rad. Antiox. VL - 11 IS - 1 SE - Short Communication DO - 10.5530/fra.2021.1.5 UR - https://antiox.org/index.php/fra/article/view/301 SP - 24-26 AB - <p><strong>Objectives: </strong>To study the antioxidant activity of methanolic and ethanolic extracts of seeds of <em>Macrotyloma uniflorum</em>. <strong>Methods: </strong>The seeds of <em>M. uniflorum </em>were extracted with methanol and ethanol and used for the phytochemical analysis and determination of antioxidant activity. The <em>in vitro </em>antioxidant activity was studied using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging methods. <strong>Result: </strong>Ethanolic extracts of <em>M. uniflorum </em>showed more phenolic content (63.48 mg GAE/g) than methanolic extract of <em>M. uniflorum </em>(45.84 mg GAE/g). In total flavonoid content analysis, methanolic and ethanolic extracts of <em>M. uniflorum </em>showed the presence of flavonoid content of 15.31 mg Rutin/g and 15.44 mg Rutin/g, respectively. In DPPH assay, methanolic and ethanolic extracts of <em>M. uniflorum </em>exhibited 50% free radical scavenging activity at 797.71 ± 34.38 μg/mL and 938.80 ± 66.05 μg/mL (mean ± standard deviation; <em>n </em>= 3), respectively. In hydroxyl radical scavenging assay, methanolic and ethanolic extracts of <em>M. uniflorum </em>exhibited 50% fee radical scavenging activity at 770.27 ± 11.64 μg/ mL and 844.94 ± 35.12 μg/mL (mean ± standard deviation; <em>n </em>= 3), respectively. Ascorbic acid exhibited potent free radical scavenging with IC50 value of 60.54 ± 5.23 μg/mL in DPPH method and 207.98 ± 14.26 μg/mL (mean ± standard deviation; <em>n </em>= 3) in hydroxyl radical scavenging method. <strong>Conclusion: </strong>Ethanolic extracts of <em>M. uniflorum </em>showed more phe­nolic content than methanolic extract of <em>M. uniflorum</em>. In both, DPPH and hydroxyl radical scavenging assay, methanolic and ethanolic extracts of <em>M. uniflorum </em>exhibited antioxidant activity at higher concentration.</p> ER -