TY - JOUR AU - Farmani, Fatemeh AU - Moein, Mahmoodreza AU - Khoshnood, Mohammad Javad AU - Sabahi, Zahra PY - 2017/10/30 Y2 - 2024/03/28 TI - Anthocyanin Isolation from Berberis integerrima Bunge Fruits and Determination of their Antioxidant Activity JF - Free Radicals and Antioxidants JA - Free Rad. Antiox. VL - 8 IS - 1 SE - Original Article DO - 10.5530/fra.2018.1.1 UR - https://antiox.org/index.php/fra/article/view/69 SP - 1-5 AB - <p><strong>Background:</strong> Anthocyanins are famous members of flavonoid group with multifaceted effect including anti-inflammatory, anti-oedema activities, antioxidant and antitumor activity. <em>Berberis integerrima</em> is candidate in treatment of gastrointestinal diseases, bleeding, fever, malaria and hepatitis. The aim of this study is to isolate anthocyanin of <em>Berberis integerrima</em> Bunge fruits (AFBI) and measurement of free-radical-scavenging activities by different methods. <strong>Materials and Methods:</strong> AFBI isolated by column chromatography. Anthocyanin content was measured by PH-differential method and antioxidant activities of AFBI quantified by various methods (DPPH, FRAP, ABTS) as well as lipid peroxidation.<strong> Results:</strong> Anthocyanin content is 14.36 ± 0.33 mg/g before purification by column and 34.51 ± 0.42 mg/g in AFBI. IC<sub>50</sub> of anthocyanin fraction before loading on column are 471.06 ± 1.8 μg/ml, 65.98 ± 0.66 μg/ml and 495.5 ± 1 μg/ml for DPPH, FRAP and ABTS assay, respectively. These IC<sub>50</sub> after loading on column are 66 ± 0.04 μg/ml, 11 ± 0.16 and 60 ± 2.1 μg/ml for DPPH, FRAP and ABTS assay, respectively. Nitric oxide scavenging values are 53 ± 0.5% and 97.04 ± 0.69% before and after using column for anthocyanin purification. The percentages of lipid peroxidation inhibition of AFBI are about 39% in first day and 95% after 10 days.<strong> Conclusion:</strong> According to results, mentioned method is efficient for anthocyanin purification and antioxidant activity increased significantly after loading sample on this column. <em>Berberis integerrima</em> fruits can be noticed as proper source of anthocyanin which able to scavenge different free radicals and protective agent against lipid peroxidation.</p> ER -