Antioxidant Activity and Phenolics Analysis by HPLC-DAD of Solanum thomasiifolium Sendtner (Solanaceae)

Authors

  • Fernanda S. Magalhães Laboratory of Phytochemistry, Department of Health, State University of Feira de Santana, Campus universitário, Av. transnordestina, 44036-900 Feira de Santana, Bahia, Brazil
  • Dayse S. A. Cassiano Laboratory of Phytochemistry, Department of Health, State University of Feira de Santana, Campus universitário, Av. transnordestina, 44036-900 Feira de Santana, Bahia, Brazil
  • Carla R. C. Branco Laboratory of Phytochemistry, Department of Health, State University of Feira de Santana, Campus universitário, Av. transnordestina, 44036-900 Feira de Santana, Bahia, Brazil
  • Tânia R. S. Silva Department of Biological Sciences, State University of Feira de Santana, Feira de Santana, Campus universitário, Av. transnordestina, 44036-900 Feira de Santana, Bahia, Brazil
  • Antonio C. S. Lins Department of Molecular Sciences, Federal Rural University of Pernambuco, Street Dom Manoel de Medeiros, s/n, Dois Irmãos, 52171-900 Recife, Pernambuco, Brazil
  • Tania M. S. Silva Department of Molecular Sciences, Federal Rural University of Pernambuco, Street Dom Manoel de Medeiros, s/n, Dois Irmãos, 52171-900 Recife, Pernambuco, Brazil
  • Alexsandro Branco Laboratory of Phytochemistry, Department of Health, State University of Feira de Santana, Campus universitário, Av. transnordestina, 44036-900 Feira de Santana, Bahia, Brazil

DOI:

https://doi.org/10.5530/fra.2014.1.4

Keywords:

Solanum thomasiifolium, Antioxidant activity, Total phenolic content, High performance liquid chromatography coupled with diode-array detector, Flavonoid

Abstract

Introduction: The genus Solanum is the most representative of the Solanaceae families and is considered one of the largest and most diverse among the Angiosperms. Chemotaxonomic studies have recognised the genus Solanum as a source of phenolic compounds, which present a wide variety of biological actions, including antioxidant activity. Methods: The hexane, ethyl acetate, ethanol and aqueous extracts of aerial parts of the Solanum thomasiifolium Sendtner were studied for antioxidant activity by using different assay in vitro such as inhibition of DPPH, superoxide anions and lipid peroxidation. The total phenolic content in the extracts was analysed by the Folin-Ciocalteu method. In addition, the active extracts were fractionated by open column chromatography containing Sephadex LH-20 and the phenolic compounds presents in the fractions were analysed by High Liquid Chromatography coupled with diode array detector (HPLC-DAD). Results: The better results were ethanolic and aqueous extracts which exhibited good scavenging activity of DPPH (IC50 = 23.9±0.7 and 28.3±3.1 μg/mL), ABTS (IC50 = 10.5±0.4 and 13.3±0.5 μg/mL), β-carotene t = 60min (83.3±0.1% and 91.3±1.5 %), and phenolic content (93.0±6.9 and 82.5±1.4 mgEAG/g). The analysis of UV profiles obtained by HPLC-DAD showed the presence of flavones and flavonols in the active fractions. Conclusion: This work shows that polar extracts of S. thomasiifolium were good antioxidant activity.

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Correlation between total phenolic content and the 1/IC50 values of DPPH and ABTS scavenging activity.

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Published

2014-02-24

How to Cite

Magalhães, F. S., Cassiano, D. S. A., Branco, C. R. C., Silva, T. R. S., Lins, A. C. S., Silva, T. M. S., & Branco, A. (2014). Antioxidant Activity and Phenolics Analysis by HPLC-DAD of Solanum thomasiifolium Sendtner (Solanaceae). Free Radicals and Antioxidants, 4(1), 15–23. https://doi.org/10.5530/fra.2014.1.4