Preparation, Characterization and Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of OXANOH

Authors

  • Nahidh W Hasaniya Department of Cardiovascular Surgery, Kaiser Permanente Medical Group, Fontana, California, USA.
  • Shyamal Premaratne [2] Hunter Holmes McGuire Veterans Administration Medical Centre, Richmond, Virginia, USA. [4] Department of Surgery, John A. Burns School of Medicine, University of Hawaii and Research Laboratory at the Queen’s Medical Centre, Honolulu, Hawaii, USA.
  • Wayne W Zhang Department of Surgery, University of Washington and VA Puget Sounds Healthcare System, Seattle,Washington, USA.
  • Aziz M Razzuk Department of Surgery, John A. Burns School of Medicine, University of Hawaii and Research Laboratory at the Queen’s Medical Centre, Honolulu, Hawaii, USA.
  • (Late) J Judson McNamara Department of Surgery, John A. Burns School of Medicine, University of Hawaii and Research Laboratory at the Queen’s Medical Centre, Honolulu, Hawaii, USA.
  • Roderick H Dashwood Director, Center for Epigenetics and Disease Prevention, Texas A and M College of Medicine, Houston, Texas, USA.

DOI:

https://doi.org/10.5530/fra.2018.2.11

Keywords:

Ischemia-reperfusion injury, Reactive oxygen species, Perfusion, Lung damage, Scanning electron microscopy

Abstract

Objective: Hydrogenation of the stable free radical, 2-Ethyl-2,5,5-trimethyl-3-oxazolidinoxyl (OXANO•), converts it to the intracellular spin trap, 2-Ethyl-1-hydroxy-2,5,5-trimethyl-3-oxazolidine (OXANOH). Methods: Due to the flammable nature of the reaction, this procedure must be carried out with extreme care. OXANO• (10 mM) was diluted in 50 ml of Krebs-Henseleit buffer (pH 7.4) and hydrogenated in a nitrogen atmosphere in the presence of Platinum (IV) Oxide Hydrate (100 mg). Results: The reaction was completed in approximately 45 min, and was confirmed by the disappearance of the three-electron paramagnetic resonance (EPR) signals (1:1:1). Additionally, the absorption spectra were measured for both OXANOH and OXANO• in the ultraviolet (UV) and visible light regions. Conclusion: OXANOH is an extremely valuable oxygen free radical spin trap due to its ability to trap both extra and intracellular free radicals in biological tissues.

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Schematic diagram of the set up for hydrogenation of OXANO• in the presence of Platinum Oxide and Nitrogen.

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Published

2018-04-01

How to Cite

Hasaniya, N. W., Premaratne, S., Zhang, W. W., Razzuk, A. M., McNamara, (Late) J. J., & Dashwood, R. H. (2018). Preparation, Characterization and Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of OXANOH. Free Radicals and Antioxidants, 8(2), 70–73. https://doi.org/10.5530/fra.2018.2.11