Comparative Assessment of the Anti-oxidant and Anti-clastogenic Activity of Morus alba Leaves

Authors

  • Anjali Raj Department of Pharmacology, JSS College of Pharmacy, JSS University, Mysuru, Karnataka,
  • Kenganora Mruthunjaya Department of Pharmacognosy, JSS College of Pharmacy, JSS University, Mysuru, Karnataka,
  • Subba Rao Venkata Madhunapantula Department of Biochemistry, Center of Excellence in Molecular Biology and Regenerative Medicine (CEMR), JSS Medical College, Mysuru, Karnataka,
  • Santhepete Nanjundaiah Manjula Department of Pharmacology, JSS College of Pharmacy, JSS University, Mysuru, Karnataka,

DOI:

https://doi.org/10.5530/fra.2017.1.18

Keywords:

Morus alba, MEMA, Hydroxyl, Phenols, Fenton reaction

Abstract

Objective: The present study was conducted to investigate the antioxidant and anti-clastogenic activity of fractions extracted from Morusalba (Moraceae) leaves. Methods: The leaves of Morus alba were sequentially extracted using n-Hexane, Petroleum Ether, Ethyl Acetate, Methanol and Water, and Total phenolic content was determined using Folin-Ciocalteau (FC) reagent. The anti-oxidant property of each fraction was determined using 2-Deoxy-D-ribose, 2, 2-Diphenyl-1-picryl-hydrazyl (DPPH) and Hydroxyl radical scavenging assay. Furthermore, anti-clastogenic potential of these fractions was determined using calf-thymus DNA in the presence of Fenton’s reagent followed by analyzing the samples by agarose gel electrophoresis. Results: All fractions showed the presence of varied amounts of total phenols and exhibited anti-oxidant activity as determined by scavenging the corresponding radicals. Among these fractions the methanol fraction (MEMA) showed the maximum phenol content as well as anti-oxidant activity. In addition MEMA could inhibit the breakage of DNA strands (anti-clastogenic activity) compared to other fractions tested. Discussion: Results of this study suggests that MEMA is likely to possess better antioxidant and anti-clastogenic activity.

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Published

2016-09-01

How to Cite

Raj, A. ., Mruthunjaya, K. ., Venkata Madhunapantula, S. R., & Manjula, S. N. (2016). Comparative Assessment of the Anti-oxidant and Anti-clastogenic Activity of Morus alba Leaves. Free Radicals and Antioxidants, 7(1), 123–127. https://doi.org/10.5530/fra.2017.1.18