Antioxidant fractions and phenolic constituents from leaves of Pluchea carolinensis and Pluchea rosea

Authors

  • Wilmer Hervet Perera Córdova Plant Molecular Biology and Biotechnology Unit, Institute of Botany B22, University of Liège, Sart Tilman, BE-4000 Liège, Belgium,
  • Jean-Noël Wauters Laboratory of Pharmacognosy, Institute of Pharmacy B36, University of Liège, Sart Tilman, BE-4000 Liège, Belgium
  • Claire Kevers Plant Molecular Biology and Biotechnology Unit, Institute of Botany B22, University of Liège, Sart Tilman, BE-4000 Liège, Belgium,
  • Michel Frédérich Laboratory of Pharmacognosy, Institute of Pharmacy B36, University of Liège, Sart Tilman, BE-4000 Liège, Belgium
  • Jacques Dommes Plant Molecular Biology and Biotechnology Unit, Institute of Botany B22, University of Liège, Sart Tilman, BE-4000 Liège, Belgium,

DOI:

https://doi.org/10.5530/fra.2014.2.1

Keywords:

Antioxidant potential, Caffeoylquinic acid derivatives, Cinnamic acids, Flavonoids, Phenolic profile

Abstract

Objective: The objective is to evaluate the antioxidant potential of several polar fractions of Pluchea carolinensis and Pluchea rosea as well as pure chemicals, some of them quantified in both species by high-performance liquid chromatography (HPLC). Methods: The antioxidant potential of polar fractions and pure chemicals were assayed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical potential methods. The phenolic content was performed by using Folin-Ciocalteu’s reagent. Specific phenolic acids and flavonoids were quantified by diode array detector-reversed phase-HPLC. Results: The highest DPPH antioxidant potential expressed in milligrams of trolox equivalents per gram of dry extract (mg TE/gDE) were frequently measured in fractions from n-butyl alcohol, i.e., 2 (192.1 ± 0.3); 6 (181.0 ± 0.1) of P. carolinensis and in fraction 7 (188.1 ± 5.5) of P. rosea while for oxygen radical scavenging capacity (mg TE/gDE) assay fraction 2 (543.0 ± 64.6) and 4 (501.4 ± 49.7) of P. carolinensis and 3 (401.3 ± 16.1) and 6 (401.3 ± 16.1) of P. rosea showed the best results. Some flavonoids and phenolic acids were also assayed; all of them showed highest oxygen radical absorbance capacity values. Conclusion: We report the antioxidant potential of polar fractions, as well as of some pure phenolics responsible of the antioxidant potential. Some phenolics were identified and quantified for the first time in both species. Apparently, caffeoylquinic acid derivatives contribute more significant to the total antioxidant potential of the extracts.

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Estimation of the contribution of the antioxidant potential of all the phenolics to the total antioxidant potentials (2,2-diphenyl-1 picrylhydrazyl and oxygen radical scavenging capacity) of the leaf extracts of Pluchea carolinensis and Pluchea rosea

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Published

2020-12-11

How to Cite

Wilmer Hervet Perera Córdova, Jean-Noël Wauters, Claire Kevers, Michel Frédérich, & Jacques Dommes. (2020). Antioxidant fractions and phenolic constituents from leaves of Pluchea carolinensis and Pluchea rosea. Free Radicals and Antioxidants, 4(2), 1–7. https://doi.org/10.5530/fra.2014.2.1