Evaluation of antioxidant and DNA nicking potential along with HPTLC fingerprint analysis of different parts of Pterospermum acerifolium (L.) Willd

Authors

  • Rathinavelusamy Paramaguru Department of Pharmaceutical Sciences, Birla Institute of Technology, Ranchi 835215, Jharkhand, India
  • Papiya Mitra Mazumder Department of Pharmaceutical Sciences, Birla Institute of Technology, Ranchi 835215, Jharkhand, India
  • Dinakar Sasmal Department of Pharmaceutical Sciences, Birla Institute of Technology, Ranchi 835215, Jharkhand, India
  • Dhananjay Kumar Department of Biotechnology, Birla Institute of Technology, Ranchi 835215, Jharkhand, India
  • Kunal Mukhopadhyay Department of Biotechnology, Birla Institute of Technology, Ranchi 835215, Jharkhand, India

Keywords:

PT257R/T plasmid , LPO assay , OH radical scavenging activity

Abstract

Objective: The present study was designed to evaluate the in vitro antioxidant and DNA nicking potential of various extracts and fractions of Pterospermum acerifolium (L.) Willd (Family: Stericuliaceae). Research design and methods: Antioxidant properties of the extracts and fractions was assayed by DPPH scavenging activity, non-site-specific and site-specific OH radical scavenging activity mediated 2-deoxy- D-ribose degradation, total antioxidant activity and lipid peroxidation assay by using rat liver homogenate. DNA nicking assay was studied by PT257R/T plasmid. Estimation of total phenolic content and total flavonoid content was done. Further HPTLC fingerprint of active fractions was performed. Results: Ethyl acetate fractions of leaves, flowers and bark exhibited potent antioxidant property and DNA protective effect compare to all the other extracts and fractions. Total phenolic and flavonoid content determination was also showed ethyl acetate fractions were rich in phenolic and flavonoid contents. HPTLC fingerprint revealed the total number of peaks present in the active ethyl acetate fractions of leaves, flowers and bark. Conclusion: The present study indicated that, ethyl acetate fractions of leaves, flowers and bark showed effective antioxidant and DNA protection activity and it could be the initiation for various other pharmacological studies on those fractions.

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HPTLC chromatogram of PALEF peak number 3 (AutoGenerated 3) scanned at 200 nm.

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Published

2013-08-23

How to Cite

Rathinavelusamy Paramaguru, Papiya Mitra Mazumder, Dinakar Sasmal, Dhananjay Kumar, & Kunal Mukhopadhyay. (2013). Evaluation of antioxidant and DNA nicking potential along with HPTLC fingerprint analysis of different parts of Pterospermum acerifolium (L.) Willd. Free Radicals and Antioxidants, 3(2), 100–106. Retrieved from https://antiox.org/index.php/fra/article/view/201