In vivo Anti-oxidant Activity of Dhatrinisha churna and Manjisthadi churna in High Fat Diet Induced Oxidative Stress

Abstract

Background: Dhatrinisha churna and Manjisthadi churna have been traditionally used in the Ayurvedic system of medicine and by traditional medical practicioners of India to treat hyperlipidemia. Objective: To evaluate the antioxidant activity of Dhatrinisha churna and Manjisthadi churna in experimental hyperlipidemic rats. Materials and Methods: Hyperlipidemia was induced by administrating exogenous cholesterol. Assessment of anti-oxidant status for liver was carried out by assay of various physiological enzymatic (like SOD, catalase, gluatathione peroxidase) and non enzymatic (GSH, lipid peroxidative indices) antioxidant. Result: Significant increase in the level of enzymes like SOD, catalase, glutathione peroxidase, GSH and decrease in lipid peroxidase were observed in drug treated animals. Conclusion: In conclusion, these observations show that Manjisthadi churna and Dhatrinisha churna exert their protective effect by improving antioxidant status and decreasing the lipid peroxidation, thus establishing them as an effective antioxidant.