Total Phenolic, Flavonoid and Alkaloid Contents, Oxidative DNA Damage Protective and Antioxidant Properties of Methanol and Aqueous Extracts of Dissotis rotundifolia Whole Plant

Authors

  • Williams Kweku Darkwah [1] Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Environmental Engineering Department, College of Environment, Hohai University, CHINA. [2] Department of Biochemistry, School of Biological Sciences, University of Cape Coast, Cape Coast, GHANA.
  • Yanhui Ao Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Environmental Engineering Department, College of Environment, Hohai University, CHINA.
  • Micheal Buenor Adinortey Department of Biochemistry, School of Biological Sciences, University of Cape Coast, Cape Coast, GHANA.
  • Alexander Weremfo Department of Biochemistry, School of Biological Sciences, University of Cape Coast, Cape Coast, GHANA.
  • Francis Kwame Abrokwah Department of Biochemistry, School of Biological Sciences, University of Cape Coast, Cape Coast, GHANA.
  • Emmanuel Afriyie [2] Department of Biochemistry, School of Biological Sciences, University of Cape Coast, Cape Coast, GHANA. [3] Department of Bioscience, University of Milan, Milan, ITALY.

DOI:

https://doi.org/10.5530/fra.2018.2.13

Keywords:

Dissotis rotundifolia whole plant, Reactive Oxygen Species (ROS), Reactive Nitrogen Species, DNA damage protecting activity and Antioxidant activity

Abstract

Background: Antioxidants present in natural sources helps to scavenge free radicals and thus, provide health benefits. The main objective for this research is to determine phenolic, flavonoid, and alkaloid contents, nitric oxide scavenging activity, hydroxyl radical scavenging activity, and DPPH-scavenging activity, ferric reducing power of the extracts and DNA damage protecting activity to evaluate the antioxidant potential of various extracts. Materials and Methods: In vitro antioxidant potential of the D. rotundifolia extract was evaluated using 1, 1‑diphenyl‑2‑picrylhydrazyl (DPPH), nitric oxide (NO), and hydroxyl (OH) radical scavenging assays. Ferric reducing power ability of the extract was also examined using tannic acid, and ascorbic acid as standard. Concentrations of plant extracts ranging from 0.02 to 0.10 mg/ml were prepared and mixed with appropriate volumes of reagents. Results: Methanol extract of D. rotundifolia exhibited higher content of phytochemical compounds (alkaloid = 12.4 mg QE/g; flavonoids = 19.3 mg QE/g; and phenols = 18 mg QE/g) at concentration 0.1 mg/ml compare to the aqueous extract (alkaloid = 9.1 mg QE/g; flavonoids = 10.5 mg QE/g; and phenols = 16.5 mg QE/g). An over-all trend found in the present study highlights the fact that the methanol extract have better antioxidant capacities (DPPH, NO, OH and FRAP) than the aqueous extract. D. rotundifolia extracts exhibited considerable protection to the damage of native supercoiled circular at concentrations, 0.1 mg/ml and 10 mg/ml. Conclusion: The study showed that the extracts can competently protect the body against oxidative stress, therefore can be used as a source of potent natural antioxidant compounds.

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Total Phenolic, Flavonoid and Alkaloid Contents, Oxidative DNA Damage Protective and Antioxidant Properties of Methanol and Aqueous Extracts of Dissotis rotundifolia Whole Plant

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Published

2018-04-01

How to Cite

Darkwah, W. K., Ao, Y., Adinortey, M. B., Weremfo, A., Abrokwah, F. K., & Afriyie, E. (2018). Total Phenolic, Flavonoid and Alkaloid Contents, Oxidative DNA Damage Protective and Antioxidant Properties of Methanol and Aqueous Extracts of Dissotis rotundifolia Whole Plant. Free Radicals and Antioxidants, 8(2), 82–88. https://doi.org/10.5530/fra.2018.2.13