Phenolic compounds from kenaf (Hibiscus cannabinus L.) seeds by ultrasound-assisted extraction

Authors

  • Yu Hua Wong Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, 56000 Kuala Lumpur, Malaysia
  • Joo Sheng Beh Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, 56000 Kuala Lumpur, Malaysia,
  • Chin Ping Tan Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia,
  • Kamariah Long Biotechnology Research Center, Malaysian Agricultural Research & Development Institute, 50774 Kuala Lumpur, Malaysia
  • Kar Lin Nyam Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, 56000 Kuala Lumpur, Malaysia,

DOI:

https://doi.org/10.5530/fra.2014.2.2

Keywords:

Antioxidant, Chromatography, Kenaf, Phenolic, Purification

Abstract

Introduction: There is great interest in the replacement of synthetic antioxidants with natural sources, especially from plant materials. Kenaf seed potentially contained phenolic compounds. Materials and Methods: Kenaf seed extract (KSE) was extracted by ultrasonic-assisted extraction. Then, the purification was carried out using silica gel column chromatography. Finally, the phenolic compounds were determined by high-performance liquid chromatography. Result: There were 11 phenolic acids identified from the purified KSE, which were identified as sinapic acid (3563.67 mg/100 g), ferulic acid (2085.55 mg/100 g), catechin hydrate (1189.02 mg/100 g), tannic acid (1032.24 mg/100 g), naringin (385.75 mg/100 g), 4-hydroxybenzoic acid (382.51 mg/100 g), gallic acid (380.18 mg/100 g), 4-hydroxybenzaldehyde (372.00 mg/100 g), protocatehuic acid (207.04 mg/100 g), syringic acid (189.13 mg/100 g), and cinnamic acid (85.51 mg/100 g). Conclusion: The KSE was found to exhibit an antioxidant capability, and it is potentially suitable to be used as an alternative source of antioxidants.

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Thin layer chromatography (TLC) profiles of five purified kenaf (Hibiscus cannabinus L.) seed extract from hexane fraction to methanol fraction with the first and second column replicate in each TLC plate. (a) Hexane fraction; (b) hexane and ethyl acetate fraction; (c) ethyl Acetate fraction; (d) ethyl acetate and methanol fraction; (e) methanol fraction

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Published

2020-12-11

How to Cite

Yu Hua Wong, Joo Sheng Beh, Chin Ping Tan, Kamariah Long, & Kar Lin Nyam. (2020). Phenolic compounds from kenaf (Hibiscus cannabinus L.) seeds by ultrasound-assisted extraction. Free Radicals and Antioxidants, 4(2), 8–12. https://doi.org/10.5530/fra.2014.2.2