Phenolic compounds from kenaf (Hibiscus cannabinus L.) seeds by ultrasound-assisted extraction
DOI:
https://doi.org/10.5530/fra.2014.2.2Keywords:
Antioxidant, Chromatography, Kenaf, Phenolic, PurificationAbstract
Introduction: There is great interest in the replacement of synthetic antioxidants with natural sources, especially from plant materials. Kenaf seed potentially contained phenolic compounds. Materials and Methods: Kenaf seed extract (KSE) was extracted by ultrasonic-assisted extraction. Then, the purification was carried out using silica gel column chromatography. Finally, the phenolic compounds were determined by high-performance liquid chromatography. Result: There were 11 phenolic acids identified from the purified KSE, which were identified as sinapic acid (3563.67 mg/100 g), ferulic acid (2085.55 mg/100 g), catechin hydrate (1189.02 mg/100 g), tannic acid (1032.24 mg/100 g), naringin (385.75 mg/100 g), 4-hydroxybenzoic acid (382.51 mg/100 g), gallic acid (380.18 mg/100 g), 4-hydroxybenzaldehyde (372.00 mg/100 g), protocatehuic acid (207.04 mg/100 g), syringic acid (189.13 mg/100 g), and cinnamic acid (85.51 mg/100 g). Conclusion: The KSE was found to exhibit an antioxidant capability, and it is potentially suitable to be used as an alternative source of antioxidants.
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![Thin layer chromatography (TLC) profiles of five purified kenaf (Hibiscus cannabinus L.) seed extract from hexane fraction to methanol fraction with the first and second column replicate in each TLC plate. (a) Hexane fraction; (b) hexane and ethyl acetate fraction; (c) ethyl Acetate fraction; (d) ethyl acetate and methanol fraction; (e) methanol fraction](https://antiox.org/public/journals/2/article_130_cover_en_US.png)