Phytochemical constituents and in vitro antioxidant capacity of Tabernaemontana catharinensis A. DC

Authors

  • Aline Augusti Boligon Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil
  • Robson Borba de Freitas Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil
  • Thiele Faccim de Brum Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil
  • Mariana Piana Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil
  • Bianca Vargas Belke Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil
  • João Batista Teixeira da Rocha Department of Chemistry, Federal University of Santa Maria, RS 97105-900, Brazil
  • Margareth Linde Athayde Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil

Keywords:

Tabernaemontana catharinensis , Apocynaceae , Antioxidant , TBARS

Abstract

Introduction: Free radicals induce numerous diseases by lipid peroxidation, protein peroxidation, and DNA damage. It has been reported that numerous plant extracts have antioxidant activities to scavenge free radicals. In this present study we determined the in vitro antioxidant capacity and quantified of total phenolics, flavonoids, tannins and alkaloids of Tabernaemontana catharinensis crude extract and fractions leaves. Methods: The antioxidant potential was evaluated by 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging and thiobarbituric acid reactive species (TBARS) methods, total phenolics content was determined using the FolineCiocalteu assay, flavonoids, tannins and alkaloids were determined by spectrophotometer. Results: Crude extract and fractions showed inhibition against TBARS, ethyl acetate was the most effective fraction (IC50 = 6.71 ± 0.19 mg/mL), subsequently by butanolic (26.15 ± 0.08 mg/mL), dichloromethane (43.25 ± 0.12 mg/mL) and crude extract (61.09 ± 0.05 mg/mL), respectively. Moreover, the DPPH assay, presented IC50 value ranged of 4.64 ± 1.25 to 27.78 ± 0.93 mg/mL. Contents of total phenols, flavonoids, tannins and alkaloids of T. catharinensis followed the order: ethyl acetate > butanolic > dichloromethane fractions > crude extract. Conclusion: The present study, we found that the crude extract and fractions of T. catharinensis showed good antioxidant activity. Among the samples tested, the ethyl acetate fraction showed better activity than others.

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Effects of different concentrations of crude extract, ethyl acetate dichloromethane, and butanolic fractions from the leaves of Tabernaemontana catharinensis on Fe(II) (10 mM)-induced TBARS production in brain homogenates. Data show means  SEM values average from 3 to 4 independent experiments performed in duplicate.

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Published

2013-08-23

How to Cite

Aline Augusti Boligon, Robson Borba de Freitas, Thiele Faccim de Brum, Mariana Piana, Bianca Vargas Belke, João Batista Teixeira da Rocha, & Margareth Linde Athayde. (2013). Phytochemical constituents and in vitro antioxidant capacity of Tabernaemontana catharinensis A. DC. Free Radicals and Antioxidants, 3(2), 77–80. Retrieved from https://antiox.org/index.php/fra/article/view/192

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