Dual effects of (þ)-catechin on hemin-induced oxidative reactions: A potential pharmacological implication

Authors

  • Nai-Hao Lu [a] Key Laboratory of Functional Small Organic Molecule, Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China [b] Jiangxi Key Laboratory of Functional Organic Molecules, Jiangxi Science and Technology Normal University, Nanchang 330013, China
  • Ying-Jie He [a] Key Laboratory of Functional Small Organic Molecule, Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China. [c] Key Laboratory of Green Chemistry, Jiangxi Province and College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, Jiangxi, 330022, China
  • Chao Chen Key Laboratory of Functional Small Organic Molecule, Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China
  • Rong Tian Key Laboratory of Green Chemistry, Jiangxi Province and College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, Jiangxi, 330022, China
  • Qiang Xiao Jiangxi Key Laboratory of Functional Organic Molecules, Jiangxi Science and Technology Normal University, Nanchang 330013, China
  • Yi-Yuan Peng [a] Key Laboratory of Functional Small Organic Molecule, Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China. [c] Key Laboratory of Green Chemistry, Jiangxi Province and College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, Jiangxi, 330022, China

Keywords:

Hemin , (þ)-Catechin , Oxidative reactions , Anti-oxidant , Pro-oxidant

Abstract

Introduction: Although the anti- and pro-oxidant properties were the well-known phenomenons for flavonoids, the influence of flavonoids on free heme-dependent redox reactions was not definitely elucidated. Methods: By spectrophotometry and Western blotting, the influence of (+)-catechin (a polyphenol identified in red wine, tea, and cocoa) on hemin-induced oxidative reactions was investigated in this study. Results: (+)-Catechin was found to act as an efficient reducing agent for the reduction of ferryl heme to ferric state, demonstrating novel anti-oxidant pathway. However, this antioxidant had the ability to trigger hemin oxidation through producing additional hydrogen peroxide (H2O2). On the other hand, the effects of (+)-catechin on hemin-H2O2-induced protein (or methylene blue) oxidation depended on the concentrations of H2O2 and antioxidant. (+)-Catechin exerted significant anti-oxidant ability when lower concentration of H2O2 was used. However, in the presence of higher concentration of H2O2, (+)-catechin at low concentrations could significantly aggravate oxidative reactions and exhibit protective effects at high concentrations. Conclusions: The anti- and pro-oxidant effects of catechin on heme-dependent redox reactions at different concentration, may provide new insights into the dietary intake and therapeutic implications of catechins with free heme and heme proteins.

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(A) Spectral changes of ferryl hemin after addition of (þ)-catechin. Ferryl hemin was generated by addition of H2O2 to hemin in a 1:1 ratio at pH 7.4. After 15 min, catalase was added (10 U) and then (þ)-catechin (20 mM, final concentration). The final hemin concentration was 20 mM. Difference spectra derived from (Inset) in which initial ferryl hemin spectrum has been set to zero. Inset: absorbance spectra taken approximately every 1 min after the addition of (þ)-catechin. (B) Spectral changes of hemin after addition of H2O2 in the presence of (þ)-catechin. Hemin (20 mM) was incubated with H2O2 (20 mM)-catechin (20 mM) at 37 C for 20 min. Difference spectra derived from (Insert) in which hemin spectrum has been set to zero. Inset: absorbance spectra taken approximately 20 min after the addition of (þ)-catechin.

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Published

2013-03-29

How to Cite

Nai-Hao Lu, Ying-Jie He, Chao Chen, Rong Tian, Qiang Xiao, & Yi-Yuan Peng. (2013). Dual effects of (þ)-catechin on hemin-induced oxidative reactions: A potential pharmacological implication. Free Radicals and Antioxidants, 3(1), 11–16. Retrieved from https://antiox.org/index.php/fra/article/view/210